Journal of Natural Remedies

Nikita Thorat ¹, Tanvi Dodiya ¹, Disha Prajapati ¹

Affiliations
1 Parul Institute of Pharmacy and Research, Parul University, Limda, Waghodia, Vadodara - 391760, Gujarat, IN

Abstract

The objective of the current study was to develop a new simple and precise High Performance Thin Layer Chromatography (HPTLC) method for standardization of three biomarkers i.e., berberine, ellagic acid and ferulic acid in Amrtadi churna. Amrtadi churna is mainly used for hyperacidity and contains dried plant parts of Gokshur, Amla and Guduchi. The method was developed and validated using precoated silica gel at 60 F254 as the stationary phase and toluene:ethyl acetate:formic acid:methanol (6:6:1.6:0.4, v/v/v/v) as the mobile phase. The detection and quantification were performed at 319 nm and the Rf value obtained was 0.35±2 for berberine, 0.5±2 for ellagic acid and 0.74±2 for ferulic acid. The method was validated as per ICH guidelines in terms of linearity, precision, specificity, accuracy and robustness.

Keywords

Amla, Gokshur, Guduchi, ICH, Standardization

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Vaishali Patil ¹, Tanvi Dodiya ²

Affiliations
1 Department of Quality Assurance, Faculty of Pharmacy, Parul University, Vadodara, Gujarat, IN
2 Department of Pharmacognosy, Faculty of Pharmacy, Parul Institute of Pharmacy & Research, Parul University, Vadodara, Gujarat, IN

Abstract

Moringa concanensis Nimmo. (Moringaceae) is a wild plant found in India. Traditionally, tribes used this plant as an antioxidant, anticancer, anticonvulsant, antimicrobial, antifertility, anti-inflammatory, antipyretic, and analgesic. In the present study, the leaves of Moringa concanensis were subjected to successive solvent extraction with five different solvents of increasing polarity, i.e., petroleum ether, chloroform, ethyl acetate, methanol, and water, followed by a phytochemical screening of each extract. The phytochemical study indicated the presence of terpenoids, steroids, cardiac glycosides, flavonoids, alkaloids, carbohydrates, proteins, tannins, and phenolics. The chloroform extract showed the presence of cardiac glycosides (cardenolides); therefore, a RP-HPLC method was developed to identify the number of constituents in the extract. The extract was recrystallized to isolate the single component and to gain the desired purity for further spectral analysis. Different spectral data from UV-Visible, Infrared, Mass, and Nuclear Magnetic Resonance spectroscopy were analyzed and the isolated chemical constituent was identified.

Keywords

Cardiac Glycosides, Infrared Spectroscopy, Mass Spectroscopy, RP-HPLC, Structure Elucidation

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Nisha Somavanshi ¹, Tanvi Dodiya ¹, Disha Prajapati ¹

Affiliations
1 Parul Institute of Pharmacy and Research, Parul University, Limda, Waghodia - 391760, Vadodara, Gujarat, IN

Abstract

In this recent study, several trials were made to develop a HPTLC method for quantification of scopoletin and piperine in the Ajmodadi formulation. HPTLC was done on pre-coated silica gel 60 F254 plates with a mobile phase of toluene:ethyl acetate:methanol:formic acid (3.9:3.9:0.3:1.7 v/v/v/v). The retardation factors for scopoletin 0.75 and for piperine was 0.86 and found a good and defined resolution peak. Densitometer analysis of scopoletin and piperine was carried out at 335 wavelength (nm). The developed method was validated as reported in ICH guidelines (Q2R1). A linearity study shows that scopoletin and piperine was linear, and the recovery studies revealed a recovery in between 98-102 % as per guideline. This method was established to be rapid, delicate, accurate and specific, thus, these methods may be used in the quantification of the drug in any polyherbal formulation.

Keywords

Ajmodadi Churna, HPTLC, Piperine, Scopoletin.

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Vaishali Patil ¹, Tanvi Dodiya ²

Affiliations
1 Department of Quality Assurance, Faculty of Pharmacy, Parul University, Vadodara - 391760, Gujarat, IN
2 Parul Institute of Pharmacy and Research, Faculty of Pharmacy, Parul University, Vadodara - 391760, Gujarat, IN

Abstract

Cardiac glycosides are the secondary metabolites that are used in atrial fibrillation and atrial flutter as it binds with the sodium-potassium ATPase pump and increases the force of contraction. There are two types of cardiac glycosides i.e. cardenolides and bufadienolides. Digoxin is a cardenolide type of cardiac glycoside. Digoxin is found in chloroform extract of Moringa concanensis leaves. In the present study, the reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated to determine the content of digoxin in chloroform extract of M. concanensis leaves from three different geographical sources. The chromatographic method was developed at 220 nm wavelength with an Acetonitrile: Water: 1% Orthophosphoric acid (50:50:0.1 %V/V/V) mobile phase. In this method, the digoxin shows linearity in the range of 10 – 50 μg/ml with a regression coefficient of 0.995. The Limit of detection (LOD) and Limit of quantitation (LOQ) was found to be 0.232 μg/ml and 0.703 μg/ml respectively.

Keywords

Chloroform Extract, Moringa concanensis, RP-HPLC Method, Soxhlet Extraction.

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